SEED PROGRAM SPECIFIC WORK INSTRUCTION - OFFICIAL SEED SAMPLING
APPENDIX I: APPROVED SAMPLING EQUIPMENT
Small - Nobbe or Double sleeve trier with slot size width 8 mm to 14 mm
Medium - Nobbe or Double sleeve trier with slot size width 15 mm to 19 mm
Large - Nobbe or Double sleeve trier with slot size width 20 mm or greater
IT IS NOT PERMISSIBLE TO USE A TRIER FOR SAMPLING ANY CROP KIND THAT IS SMALLER THAN THAT STATED IN THIS TABLE UNLESS THE SAMPLER PROVIDES EVIDENCE THAT THE WIDTH OF THE OPENING IS, AT A MINIMUM, TWO TIMES THE DIAMETER OF THE SEED.
| Crop Kind | Trier Size |
|---|---|
| Alfalfa | Small |
| Alkaligrass, Weeping | Small |
| Artichoke, cardoon | Medium |
| Asparagus | Medium |
| Barley | Large |
| Bean, Field | Large |
| Bean, Scarlet Runner | Large |
| Bean, Garden | Large |
| Bean, Mung | Medium |
| Bean, Lima | Large |
| Bean, Broad | Large |
| Bean, Faba | Large |
| Beet | Medium |
| Bentgrass | Small |
| Bluegrass | Small |
| Brassica spp. | Small |
| Bromegrass, Sweet | Large |
| Bromegrass, Meadow | Medium |
| Bromegrass, Smooth | Medium |
| Buckwheat | Medium |
| Canarygrass, Reed | Small |
| Canarygrass | Medium |
| Cantaloupe | Large |
| Carrot | Small |
| Celery, celeriac | Small |
| Chervil, Turnip-rooted | Medium |
| Chickpea | Large |
| Chicory, Cultivated | Small |
| Chives | Small |
| Citron, Watermelon | Small |
| Clover, alsike | Small |
| Clover, crimson | Small |
| Clover, red | Small |
| Clover, hop | Small |
| Clover, Persian | Small |
| Clover, strawberry | Small |
| Clover, subterranean | Small |
| Clover, sweet | Small |
| Clover, white | Small |
| Corn | Large |
| Cornsalad | Small |
| Cowpea | Large |
| Cress, Water | Small |
| Cress, Garden | Small |
| Crested dogtail | Medium |
| Cucumber | Large |
| Dandelion, Cultivated | Medium |
| Dill | Medium |
| Eer, spikelets | Large |
| Eer, caryopses | Medium |
| Eggplant | Small |
| Emmer | Large |
| Endive | small |
| Fescues | Medium |
| Flax | Medium |
| Foxtails | Medium |
| Gherkin | Medium |
| Leek | Small |
| Lentil | Medium |
| Lespedeza spp. | Small |
| Lettuce, celtus | Medium |
| Lupine, Blue and yellow | Large |
| Lupine, White | Large |
| Mangel | Medium |
| Medick, Black | Small |
| Milk-vetch, Cicer | Small |
| Millet, Proso | Small |
| Millet, Foxtail or Italian | Small |
| Millet, Pearl | Medium |
| Millet, Japanese | Medium |
| Muskmelon | Large |
| Mustard, black | Small |
| Mustard, Oriental or Indian | Small |
| Mustard, white | Small |
| Oat | Large |
| Oatgrass, Tall | Medium |
| Okra | Medium |
| Onion | Small |
| Orchardgrass | Medium |
| Parsley | Medium |
| Parsnip | Medium |
| Pea | Large |
| Peanut | Large |
| Pepper | Medium |
| Pumpkin | Large |
| Radish | Medium |
| Rampion | Small |
| Rape, forage | Small |
| Rapeseed, Oilseed Rape including Canola |
Small |
| Redtop | Small |
| Rhubarb | Medium |
| Rye | Large |
| Ryegrass | Medium |
| Safflower | Medium |
| Sage | Small |
| Sainfoin | Medium |
| Salsify | Large |
| Savory | Small |
| Sorghum spp. | Medium |
| Sorrel, Garden | Small |
| Soybean | Large |
| Soybean, Natto-type | Medium |
| Spelt, caryopses | Medium |
| Spelt, spikelets | Large |
| Spinach, New Zealand | Large |
| Spinach | Large |
| Squash | Large |
| Sudan grass | Medium |
| Sunflower | Large |
| Sweetclover | Small |
| Swiss chard | Medium |
| Thyme | Small |
| Timothy | Small |
| Tobacco | Small |
| Tomato | Small |
| Trefoil, Bird's-foot | Small |
| Triticale | Large |
| Vetch, Crown | Small |
| Vetch, Kidney | Small |
| Vetches | Medium |
| Wheat, Durum | Large |
| Wheat, Common | Medium |
| Wheatgrass | Medium |
| Wheatgrass, Crested | Medium |
| Wild-rye | Medium |
| Broccoli, Brussel Sprouts, Cabbage | Small |
| Kale, Kohlrabi, Rutabaga (Swede), Turnip | Small |
| Spinach mustard, Vegetable Marrow | Small |
| Very chaffy grasses | Hand or stream sampling |
| Seed Mixtures, forage, lawn and turf, ground cover | Based on size of largest component in the mixture |
Note
- For horizontal probing of large containers, Nobbe and sleeve triers must reach to the centre of the container if the container can be probed from both sides.
- Probing of very chaffy grasses in large containers is difficult. Sampling is best done by stream sampling as the container is filled.
- Where sampling kinds other than those in this table, refer back to Section 7.2 (Paragraph 2) for specific guidance on which trier size to use for such types of seed.
- When sampling vertically, the official seed sampler must use the partitioned trier.
APPENDIX II: SAMPLING INTENSITY
A representative sample of the seed lot must be obtained as described below:
The sampling intensities listed below are from the International Seed Testing Rules, 2.6.2
BAGGED SEED
| Lot Size | Number of Primary Samples to Be Taken |
|---|---|
| 1 - 4 containers | Three (3) primary samples from each container |
| 5 - 8 containers | Two (2) primary samples from each container |
| 9 - 15 containers | One (1) primary sample from each container |
| 16 - 30 containers | Fifteen (15) primary samples from the seed lot |
| 31 - 59 containers | Twenty (20) primary samples from the seed lot |
| 60 or more containers | Thirty (30) primary samples from the seed lot |
For seed lots in containers smaller than 15 kg capacity, containers shall be combined into sampling units not exceeding 100 kg and the sampling unit shall be regarded as the minimum requirement.
BULK CONTAINERS
| Lot Size | Number of Primary Samples to be Taken |
|---|---|
| up to 500 kg | At least five (5) primary samples. |
| 501-3,000 kg | One (1) primary sample for each 300 kg, but not less than five. |
| 3,001-20,000 kg | One (1) primary sample for each 500 kg, but not less than 10. |
| 20,001 kg and above | One (1) primary sample for each 700 kg but not less than 40. |
In all cases, when sampling a seed lot of up to 15 containers, all containers must be sampled, and the same number of primary samples shall be taken from each container.
Example:
The seed lot is 6 containers of 1200 kg
each.
It is required that one primary sample per 500 kg be taken
15 primary samples from 6 containers = 2. 5 primary samples from each container which must be rounded up to 3. Therefore, 3 primary samples must be taken from each container
SAMPLING SMALL PACKAGES
When sampling seed in small packages (for export purposes), the sampling intensity stated in the ISTA Rules are applicable.
Example:
(The sampling intensity table for packages up to 100 kg is applicable)
Seed lot size is 2000 kg packaged in 4 kg boxes (500 boxes at 4 kg = 2000 kg)
Combine the containers into units that are 100 kg.
 |
(Each unit is considered to be a container for sampling purposes) |
For sampling purposes, this seed lot is considered to be 20 containers (units) which would require 15 primary samples in total to be drawn.
The seed lot shall be divided into 20 units. Each unit will contain 25 boxes (100/4 kg = 25 containers). Fifteen (15) primary samples in total are to be drawn from the 20 units.
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