Plant Pest Containment Level 1 Self Assessment Checklist

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The information provided in this checklist will help you evaluate the physical and operational components of your facility as described in the Containment Standards for Facilities Handling Plant Peststo determine if your facility meets the Plant Pest Containment level 1 (PPC-1) requirements.

Facility:

  • Postal Address:
  • Tel:
  • Fax:
  • E-mail:
  • Website:

Room(s) / Building(s):

Type of Facility:

check box Greenhouse check box Laboratory check box Other

Contact Information:

1) Facility Supervisor

  • Name:
  • Title:
  • Department:
  • Address:
  • Phone number:
  • Fax number:
  • E-mail:
  • Language preference: check box English / check box Français

Signature: Date:

2) Biosafety Officer (or equivalent)

  • Name:
  • Title:
  • Department:
  • Address:
  • Phone number:
  • Fax number:
  • E-mail:
  • Language preference: check box English / check box Français

Signature: Date:

Physical and Operational Components

The following tables describe physical and operational containment requirements for Plant Pest Containment (PPC) Level 1 facilities (e.g. laboratories, greenhouses, screenhouses) working with plant pests.
The following symbols are used: Required Required Recommended Recommended

Note: Recommended items are optional, depending upon the nature of the pest(s) requiring containment.

Physical Components
Structure, Location and Access
Q# Reference Components PPC-1 Yes No Comments
1 3.7.1.2 Is signage installed on entry doors within the containment zone indicating containment level, contact information, and entry requirements? Recommended check box check box  
2 3.7.1.3 Is entrance to the containment zone via self-closing and lockable doors? Recommended check box check box  
3 3.7.1.4 Is restricted access to the containment zone ensured through a controlled access system (e.g. electronic access card, code or equivalent)? Recommended check box check box  
4 3.7.1.15a Can emergency exits be opened only from inside the containment zone? Recommended check box check box  
5 3.7.1.15b Are emergency exits alarmed? Recommended check box check box  
6 3.7.1.15c Are emergency exits identified as “Emergency Exit Only”? Recommended check box check box  
7 3.7.1.16 Are dedicated laboratory clothing and personal protective equipment stored separately from street clothing? Recommended check box check box  

 

Physical Components
Surface Finishes and Casework
Q# Reference Components PPC-1 Yes No Comments
8 3.7.2.4a Are bench tops non-absorptive, impervious to water, and resistant to acids, alkalis, organic solvents and moderate heat? Recommended check box check box  
9 3.7.2.4b Are backsplashes installed tight to wall and sealed at wall-bench junction? Recommended check box check box  

 

Physical Components
Containment Perimeter
Q# Reference Components PPC-1 Yes No Comments
10 3.7.3.1 Is an autoclave or other validated means of waste treatment/disposal located within the containment zone, or if not available in the containment zone, are procedures in place to safely transport waste for treatment/disposal? Required check box check box  Specify:
check box within PPC-1 zone
check box outside PPC-1 zone
Method of waste treatment (include time and temperature parameters):
11 3.7.3.3 Is the autoclave equipped with a cycle log recorder to record time, temperature, and pressure? Recommended check box check box  
12 3.7.3.7 Is the containment zone screened or sealed (including all windows, supply/exhaust air ducts)? Required check box check box  

 

Physical Components
Facility Services
Q# Reference Components PPC-1 Yes No Comments
13 3.7.5.1 Is a hand washing sink located within the containment zone and near the point of exit? Recommended check box check box  
14 3.7.5.3 Are appropriate primary containment devices available (e.g. BSCs) to minimize the potential contamination of the containment zone? Recommended check box check box  
15 3.7.5.4 Are emergency eyewash facilities provided in the containment zone in accordance with activities and applicable regulations (i.e. ANSI Z358.1-2004)? Required check box check box  
16 3.7.5.5 Is an emergency shower provided in the containment zone in accordance with activities and applicable regulations (i.e. ANSI Z358.1-2004)? Required check box check box  
17 3.7.5.9 Are soil traps installed in drains? Required check box check box  

 

Physical Components
Greenhouses – if facility is not a greenhouse, proceed to Q#24
Q# Reference Components PPC-1 Yes No Comments
18 3.7.1.18 Are greenhouses designed to withstand extremes of local weather and anticipated maximum snow and ice loads, as well as wind, windborne debris and hail? Recommended check box check box  
19 3.7.2.5 Are greenhouse floors impervious to water and easy to clean (e.g. concrete)? Recommended check box check box  
20 3.7.3.8 Are spare greenhouse window panels, emergency glazing and screening stored nearby for emergency repairs? Recommended check box check box  
21 3.7.4.16 Are greenhouse vents and greenhouse HVAC penetrations screened with appropriate mesh screening to prevent pest escape? Required check box check box  
22 3.7.4.18 Is the greenhouse ventilation system designed to allow for greenhouse fumigation and pesticide applications? Required check box check box  
23 4.1.5.21 Are greenhouse personnel who apply pesticides appropriately trained and protected? Required check box check box  

 

Operational Components
Access
Q# Reference Components PPC-1 Yes No Comments
24 4.1.1 Is access to containment zone and support areas restricted to authorized personnel only? Required check box check box  

 

Operational Components
Documentation
Q# Reference Components PPC-1 Yes No Comments
25 4.1.2.2 Is there an up-to-date inventory of all imported plant material and plant pests?
Note: records should also be kept of all inoculations or infestations of plant material and the movement of plant material and plant pests into or out of containment.		 Required check box check box  

 

Operational Components
Training
Q# Reference Components PPC-1 Yes No Comments
26 4.1.3a Is personnel provided with training on pest-associated hazards and the precautions necessary to prevent the release of contained pests? Required check box check box  
27 4.1.3b Is personnel required to show evidence that they know and understand the required precautions? Required check box check box  
28 4.1.3c Is the training documented? Required check box check box  
29 4.1.3d Are refresher and retraining programs implemented? Required check box check box  

 

Operational Components
Personal Protective Equipment
Q# Reference Components PPC-1 Yes No Comments
30 4.1.4.1 Are personnel and visitors entering the containment zone required to wear appropriate, properly fastened, protective clothing? Recommended check box check box  
31 4.1.4.2 Is protective clothing removed prior to exiting the containment zone? Required check box check box  

 

Operational Components
Work Practices
Q# Reference Components PPC-1 Yes No Comments
32 4.1.5.2 Are all organisms rendered non-viable prior to disposal? Required check box check box Specify method:
33 4.1.5.3 Are good laboratory practices employed to prevent the escape of pests? Required check box check box  
34 4.1.5.4 Are doors kept closed to reduce the potential movement of plant pests? Required check box check box  
35 4.1.5.5 Is eating, chewing gum, drinking, smoking, storing of food and utensils, storing of personal belongings, applying cosmetics, and inserting or removing contact lenses prohibited in the containment zone?
Note: the wearing of contact lenses is recommended only when other forms of corrective eyewear are not suitable.		 Recommended check box check box  
36 4.1.5.6 Is long hair tied back or restrained so that it cannot come into contact with hands, specimens, containers or equipment? Required check box check box  
37 4.1.5.7 Are all pests and materials in a containment zone treated in accordance with the highest containment requirement for that area (e.g. if PPC-1 and PPC-2 pests are in the same room, PPC-2 practices must be followed)? Required check box check box  
38 4.1.5.8 Are all pests and material infested or suspected of being infested with a pest moved or transported in containers that are secure, leak-proof and not easily broken?
Note: the containers may only be opened within a facility that provides the appropriate containment level for the pest in question.		 Required check box check box  
39 4.1.5.9 Are all work areas within a containment zone, including dedicated clerical work stations, clean and tidy?
Note: storage of materials should be minimized, and paperwork should be done outside of containment zones if this presents a risk of disseminating pests.		 Required check box check box  
40 4.1.5.11 Are cultures stored in sealed, preferably break-resistant, containers such as screw-top vials?
Note: cultures are to be clearly identified and dated. Where possible, petri dish cultures of sporulating fungi should be sealed with stretch film.		 Required check box check box  
41 4.1.5.12 Are contaminated materials and equipment properly cleaned and decontaminated before leaving the facility for servicing or disposal? Required check box check box Specify method of decontamination:
42 4.1.5.13 Are all unintentionally introduced pests rendered non-viable, including those contaminating cultures, as soon as they are detected? Required check box check box  
43 4.1.5.15 Are disinfectants effective against the organisms in use available at all times when plant pests are handled or stored? Required check box check box Specify disinfectant used and contact time:
44 4.1.5.16 Are sanitation practices implemented when working with plants and plant pests?
Note: refer to page 37 of the Containment Standards for Facilities Handling Plant Pests for more details.		 Recommended check box check box  
45 4.1.5.18 Are autoclaves used for decontamination monitored regularly using biological indicators to ensure efficacy (e.g. consider weekly or monthly monitoring, depending on the frequency of use of the autoclave).
Note: monitoring records must be kept for three years.		 Required check box check box  
46 4.1.5.19a Are losses of containment reported immediately to the laboratory supervisor and remedied as soon as possible? Required check box check box  
47 4.1.5.19b Are written reports of such incidents maintained for three years, and the results of incident investigations used for continuing education? Required check box check box  
48 4.1.5.20 Is an effective bird, rodent, weed and plant pest control program maintained to prevent entry and eliminate undesired pests from the containment zone? Required check box check box  

 

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